Transport of gold nanoparticles through plasmodesmata and precipitation of gold ions in woody poplar.
Identifieur interne : 001F87 ( Main/Exploration ); précédent : 001F86; suivant : 001F88Transport of gold nanoparticles through plasmodesmata and precipitation of gold ions in woody poplar.
Auteurs : Guangshu Zhai [États-Unis] ; Katherine S. Walters [États-Unis] ; David W. Peate [États-Unis] ; Pedro J J. Alvarez [États-Unis] ; Jerald L. Schnoor [États-Unis]Source :
- Environmental science & technology letters [ 2328-8930 ] ; 2014.
Abstract
Poplar plants (Populus deltoides × nigra, DN-34) were used as a model to explore vegetative uptake of commercially available gold nanoparticles (AuNPs) and their subsequent translocation and transport into plant cells. AuNPs were directly taken up and translocated from hydroponic solution to poplar roots, stems and leaves. Total gold concentrations in leaves of plants treated with 15, 25 and 50 nm AuNPs at exposure concentrations of 498±50.5, 247±94.5 and 263±157 ng/mL in solutions were: 0.023±0.006, 0.0218±0.004 and 0.005±0.0003 µg/g dry weight, respectively, which accounted for 0.05, 0.10 and 0.03%, respectively, of the total gold mass added. The presence of total gold in plant tissues was measured by inductively coupled plasma mass spectrometry, while AuNPs were observed by transmission electron microscopy in plant tissues. In solution, AuNPs were distinguished from Au(III) ions by membrane separation and centrifugation. AuNPs behaved conservatively inside the plants and were not dissolved into gold ions. On the other hand, Au(III) ions were taken up and reduced into AuNPs inside whole plants. AuNPs were observed in the cytoplasm and various organelles of root and leaf cells. A distinct change in color from yellow to pink was observed as Au(III) ions were reduced and precipitated in hydroponic solution. The accumulation of AuNPs in the plasmodesma of the phloem complex in root cells clearly suggests ease of transport between cells and translocation throughout the whole plant, inferring the potential for entry and transfer in food webs.
DOI: 10.1021/ez400202b
PubMed: 25386566
PubMed Central: PMC4224293
Affiliations:
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Walters</name><affiliation wicri:level="4"><nlm:affiliation>Central Microscopy Research Facility, The University of Iowa, Iowa City, IA, 52242, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Central Microscopy Research Facility, The University of Iowa, Iowa City, IA, 52242</wicri:regionArea><orgName type="university">Université de l'Iowa</orgName><placeName><settlement type="city">Iowa City</settlement><region type="state">Iowa</region></placeName></affiliation></author><author><name sortKey="Peate, David W" sort="Peate, David W" uniqKey="Peate D" first="David W" last="Peate">David W. Peate</name><affiliation wicri:level="4"><nlm:affiliation>Department of Earth & Environmental Sciences, The University of Iowa, Iowa City, IA, 52242, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Earth & Environmental Sciences, The University of Iowa, Iowa City, IA, 52242</wicri:regionArea><orgName type="university">Université de l'Iowa</orgName><placeName><settlement type="city">Iowa City</settlement><region type="state">Iowa</region></placeName></affiliation></author><author><name sortKey="Alvarez, Pedro J J" sort="Alvarez, Pedro J J" uniqKey="Alvarez P" first="Pedro J J" last="Alvarez">Pedro J J. Alvarez</name><affiliation wicri:level="2"><nlm:affiliation>Department of Civil and Environmental Engineering, Rice University, Houston, TX, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Civil and Environmental Engineering, Rice University, Houston, TX</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Schnoor, Jerald L" sort="Schnoor, Jerald L" uniqKey="Schnoor J" first="Jerald L" last="Schnoor">Jerald L. Schnoor</name><affiliation wicri:level="4"><nlm:affiliation>Department of Civil and Environmental Engineering and IIHR Hydroscience and Engineering, The University of Iowa, Iowa City, IA, 52242, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Civil and Environmental Engineering and IIHR Hydroscience and Engineering, The University of Iowa, Iowa City, IA, 52242</wicri:regionArea><orgName type="university">Université de l'Iowa</orgName><placeName><settlement type="city">Iowa City</settlement><region type="state">Iowa</region></placeName></affiliation></author></analytic><series><title level="j">Environmental science & technology letters</title><idno type="ISSN">2328-8930</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Poplar plants (<i>Populus deltoides × nigra, DN-34</i>) were used as a model to explore vegetative uptake of commercially available gold nanoparticles (AuNPs) and their subsequent translocation and transport into plant cells. AuNPs were directly taken up and translocated from hydroponic solution to poplar roots, stems and leaves. Total gold concentrations in leaves of plants treated with 15, 25 and 50 nm AuNPs at exposure concentrations of 498±50.5, 247±94.5 and 263±157 ng/mL in solutions were: 0.023±0.006, 0.0218±0.004 and 0.005±0.0003 µg/g dry weight, respectively, which accounted for 0.05, 0.10 and 0.03%, respectively, of the total gold mass added. The presence of total gold in plant tissues was measured by inductively coupled plasma mass spectrometry, while AuNPs were observed by transmission electron microscopy in plant tissues. In solution, AuNPs were distinguished from Au(III) ions by membrane separation and centrifugation. AuNPs behaved conservatively inside the plants and were not dissolved into gold ions. On the other hand, Au(III) ions were taken up and reduced into AuNPs inside whole plants. AuNPs were observed in the cytoplasm and various organelles of root and leaf cells. A distinct change in color from yellow to pink was observed as Au(III) ions were reduced and precipitated in hydroponic solution. The accumulation of AuNPs in the plasmodesma of the phloem complex in root cells clearly suggests ease of transport between cells and translocation throughout the whole plant, inferring the potential for entry and transfer in food webs.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">25386566</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">2328-8930</ISSN><JournalIssue CitedMedium="Print"><Volume>1</Volume><Issue>2</Issue><PubDate><Year>2014</Year><Month>Feb</Month><Day>11</Day></PubDate></JournalIssue><Title>Environmental science & technology letters</Title><ISOAbbreviation>Environ Sci Technol Lett</ISOAbbreviation></Journal><ArticleTitle>Transport of gold nanoparticles through plasmodesmata and precipitation of gold ions in woody poplar.</ArticleTitle><Pagination><MedlinePgn>146-151</MedlinePgn></Pagination><Abstract><AbstractText>Poplar plants (<i>Populus deltoides × nigra, DN-34</i>) were used as a model to explore vegetative uptake of commercially available gold nanoparticles (AuNPs) and their subsequent translocation and transport into plant cells. AuNPs were directly taken up and translocated from hydroponic solution to poplar roots, stems and leaves. Total gold concentrations in leaves of plants treated with 15, 25 and 50 nm AuNPs at exposure concentrations of 498±50.5, 247±94.5 and 263±157 ng/mL in solutions were: 0.023±0.006, 0.0218±0.004 and 0.005±0.0003 µg/g dry weight, respectively, which accounted for 0.05, 0.10 and 0.03%, respectively, of the total gold mass added. The presence of total gold in plant tissues was measured by inductively coupled plasma mass spectrometry, while AuNPs were observed by transmission electron microscopy in plant tissues. In solution, AuNPs were distinguished from Au(III) ions by membrane separation and centrifugation. AuNPs behaved conservatively inside the plants and were not dissolved into gold ions. On the other hand, Au(III) ions were taken up and reduced into AuNPs inside whole plants. AuNPs were observed in the cytoplasm and various organelles of root and leaf cells. A distinct change in color from yellow to pink was observed as Au(III) ions were reduced and precipitated in hydroponic solution. The accumulation of AuNPs in the plasmodesma of the phloem complex in root cells clearly suggests ease of transport between cells and translocation throughout the whole plant, inferring the potential for entry and transfer in food webs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhai</LastName><ForeName>Guangshu</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Civil and Environmental Engineering and IIHR Hydroscience and Engineering, The University of Iowa, Iowa City, IA, 52242, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Walters</LastName><ForeName>Katherine S</ForeName><Initials>KS</Initials><AffiliationInfo><Affiliation>Central Microscopy Research Facility, The University of Iowa, Iowa City, IA, 52242, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peate</LastName><ForeName>David W</ForeName><Initials>DW</Initials><AffiliationInfo><Affiliation>Department of Earth & Environmental Sciences, The University of Iowa, Iowa City, IA, 52242, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alvarez</LastName><ForeName>Pedro J J</ForeName><Initials>PJ</Initials><AffiliationInfo><Affiliation>Department of Civil and Environmental Engineering, Rice University, Houston, TX, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schnoor</LastName><ForeName>Jerald L</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Department of Civil and Environmental Engineering and IIHR Hydroscience and Engineering, The University of Iowa, Iowa City, IA, 52242, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>P42 ES013661</GrantID><Acronym>ES</Acronym><Agency>NIEHS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Environ Sci Technol Lett</MedlineTA><NlmUniqueID>101628367</NlmUniqueID></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25386566</ArticleId><ArticleId IdType="doi">10.1021/ez400202b</ArticleId><ArticleId IdType="pmc">PMC4224293</ArticleId><ArticleId IdType="mid">NIHMS557381</ArticleId></ArticleIdList><pmc-dir>nihms</pmc-dir>
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Alvarez</name><name sortKey="Peate, David W" sort="Peate, David W" uniqKey="Peate D" first="David W" last="Peate">David W. Peate</name><name sortKey="Schnoor, Jerald L" sort="Schnoor, Jerald L" uniqKey="Schnoor J" first="Jerald L" last="Schnoor">Jerald L. Schnoor</name><name sortKey="Walters, Katherine S" sort="Walters, Katherine S" uniqKey="Walters K" first="Katherine S" last="Walters">Katherine S. Walters</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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